Anup Kumar Singh

Portrait of  Anup Kumar Singh

  • Title
    Associate Director for Engineering
  • Email
  • Phone
    (925) 423-5435
  • Organization
    Not Available

ANUP SINGH is the Associate Director for Engineering at Lawrence Livermore National Laboratory (LLNL). In this role, he leads an organization of approximately 2,300 engineering professionals with responsibility for the full breadth of the Laboratory's engineering needs including research, platforms, and services.

Singh joins the Laboratory from Sandia National Laboratories (SNL), where he served as director of the Center for Biological and Engineering Sciences, managing critical capabilities in support of Sandia's Energy & Homeland Security, Global Security, Nuclear Deterrence and Advanced Science and Technology portfolios. In 2020 he served on a special assignment coordinating COVID-19 R&D, serving as the SNL representative on the Department of Energy's (DOE) National Virtual Biotechnology Laboratory (NVBL) team and assisting with operational response efforts at Sandia. He won two 2019 Secretary of Energy Achievement Honor Awards for his contributions as a member of the NVBL and DOE National Laboratories' COVID-19 Clinical Testing teams.

Singh also holds an appointment as adjunct professor at the University of Texas Medical Branch in Galveston and serves as the director of Microfluidics at the Joint BioEnergy Institute, a DOE-funded Bioenergy Research Center. He has served on numerous review and advisory panels including the Department of Defense's Defense Science Board, the National Research Council's Chemical Science and Technology panel, the National Institutes of Health's Instrumentation and Systems Development Study Section and many review panels for the National Science Foundation and DOE.

He is internationally recognized for his scientific leadership in microfluidics and has published more than 100 peer-reviewed publications, delivered more than 150 presentations at national and international conferences, and his inventions have led to more than 40 patents and patent applications. Many of his inventions have been licensed to companies engaged in medical diagnostics and sensor technology development.

Panels and Advisory Boards (last 10 years)

  • Nominated to serve on Defense Science Board study on Emerging Biotechnologies and National Security, Dec 2020
  • Board member- California Technology Council, 2018-present
  • 5-year review panel for LBNL's Office of National & Homeland Security, 2019
  • Judge, Annual Postdoc Career Development Day Research Slam, LLNL, 2019
  • DoD's Defense Science Board task force on Preventing WMD Threat, 2015-2016
  • Standing member of NIH's Instrumentation and Systems Development (ISD), 2012-2018
  • National Research Council's Chemical Science and Technology panel, 2009
  • NSF Review Panel for Nanoscale Science and Engineering Center at Ohio State Univ, 2008- 2014
  • NIH Special Emphasis panels (NCI, NIAID, NIDA, NIBIB, NHGRI)
  • Reviewer for DOE, NIH and DoD Small Business Innovation Research (SBIR) proposals
  • National Academy of Engineering sponsored Indo-American Frontiers of Engineering Symposium (2010)

Professional Society Roles

  • Organizer, Making Bioweapons Obsolete Workshops- featuring ASPR/HHS Dr. Robert Kadlec, Nobel Laurate Jennifer Doudna, Hon. Andrew Weber (former DoD Asst Sec), and others (2019-2020).
  • Technical Program Committee Member, MicroTAS Annual Meeting 2014, San Antonio, TX.
  • Co-Organizer, Single Cell and Single Molecule Imaging and Biochemistry Conference, 2014, University of New Mexico, Albuquerque, NM.
  • Technical Program Committee Member and Session Chair, MicroTAS Annual Meeting 2013, Freiburg, Germany.
  • Co-Organizer, Jan 2013, Understanding Cell Behavior through Single Cell & Molecular Biology Conference, Albuquerque, NM.
  • Chair, 2011, Point-of-Care Diagnostics Session, Lab-On-A-Chip World Congress, South San Francisco, CA.
  • Organizer, 2009 American Electrophoresis Society Annual Meeting, Nashville, TN.
  • Co-chair, Electrofluidics Symposium, Materials Research Society 2009 Spring Meeting, San Francisco, CA.
  • Session organizer and Chair, Microdevices in Separations, American Institute of Chemical Engineering Annual Meeting, Nashville (2009), Philadelphia (2008), Salt Lake City (2007).


  1. #10,731,205 | Microfluidic platform for multiplexed detection in single cells and methods thereof | Aug 4, 2020
  2. #10,627,366 | Fluid delivery manifolds and microfluidic systems |Apr 21, 2020
  3. #10,590,477 | Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid | Mar 17, 2020
  4. #10,240,180 | Multiplexed screening of enzyme activities using nanostructure-initiator mass spectrometry |Mar 26, 2020
  5. #10,131,903 | Microfluidic platform for synthetic biology applications | Nov 20, 2018
  6. #9,957,554 | Microfluidic platform for multiplexed detection in single cells and methods thereof | May 1, 2018
  7. #9,803,238 | Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid |Oct. 31, 2017
  8. #9,766,230 | System and method for detecting components of a mixture including a valving scheme for competition assays | Sept. 19, 2017
  9. #9,702,871 | System and method for detecting components of a mixture including a valving scheme for competition assays |July 11, 2017
  10. #9,579,649 | Fluid delivery manifolds and microfluidic systems |Feb. 28, 2017
  11. #9,409,357 | Devices, systems, and methods for microscale isoelectric fractionation | Aug. 9, 2016
  12. #9,244,065 | Systems, devices, and methods for agglutination assays using sedimentation |Jan. 26, 2016
  13. #9,201,069 | Microfluidic devices and methods including porous polymer monoliths | Dec. 1, 2015
  14. #9,186,668 | Microfluidic devices, systems, and methods for quantifying particles using centrifugal force | Nov. 17, 2015
  15. #9,005,417 | Devices, systems, and methods for microscale isoelectric fractionation | Apr. 14, 2015
  16. #9,002,654 | Multi-analyte analysis of saliva biomarkers as predictors of periodontal and pre- implant disease | Apr. 7, 2015
  17. #8,961,766 | Microchannel gel electrophoretic separation systems and methods for preparing and using | Feb. 24, 2015
  18. #8,945,914 | Devices, systems, and methods for conducting sandwich assays using sedimentation | Feb. 3, 2015
  19. #8,911,606 | Dielectrokinetic chromatography devices | Dec. 16, 2014
  20. #8,871,496 | Methods, microfluidic devices, and systems for detection of an active enzymatic agent | Oct. 28, 2014
  21. #8,728,290 | Microfluidic device having an immobilized pH gradient and page gels for protein separation and analysis | May 20, 2014
  22. #8,703,058 | Microfluidic devices and methods including porous polymer monoliths | Apr. 22, 2014
  23. #8,702,946 | Dielectrokinetic chromatography and devices thereof | Apr. 22, 2014
  24. #8,524,060 | Microchannel gel electrophoretic separation systems and methods for preparing and using | Sept. 3, 2013
  25. #8,394,312 | Method for forming polymerized microfluidic devices | Mar. 12, 2013
  26. #8,329,016 | Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis | Dec. 11, 2012
  27. #8,163,154 | Method for voltage-gated protein fractionation | Apr. 24, 2012
  28. #8,047,829 | Method for forming polymerized microfluidic devices | Nov. 1, 2011
  29. #7,999,937 | Microfluidic devices and methods for integrated flow cytometry | Aug. 16, 2011
  30. #7,931,792 | System for concentrating and analyzing particles suspended in a fluid | Apr. 26, 2011
  31. #7,828,948 | Preconcentration and separation of analytes in microchannels | Nov. 9, 2010
  32. #7,754,077 | Dialysis membrane for separation on microchips | July 13, 2010
  33. #7,534,334 | Apparatus and method for concentrating and filtering particles suspended in a fluid | May 19, 2009
  34. #7,534,315 | Method for dialysis on microchips using thin porous polymer membrane | May 19, 2009
  35. #7,344,681 | Planar micromixer | March 18, 2008
  36. #7,264,723 | Dialysis on microchips using thin porous polymer membranes | Sept. 4, 2007
  37. #7,014,747 | Dielectrophoretic systems without embedded electrodes | March 21, 2006
  38. #6,495,352 | Sol-gel method for encapsulating molecules | Dec. 17, 2002
  39. #6,428,666 | Electrokinetic concentration of charged molecules | Aug. 6, 2002
  40. #5,494,803 | Immunodiagnostic assay using liposomes carrying labels thereof on outer liposome surface | Feb. 27, 1996

March 1995: Ph.D. in Chemical Engineering, North Carolina State University, Raleigh, NC

May 1990: B.Tech., Chemical Engineering, Indian Institute of Technology, Bombay, India. Recipient of the National Talent Search Scholarship

  1. Sebastian Palluk, Daniel H Arlow, Tristan De Rond, Sebastian Barthel, Justine S Kang, Rathin Bector, Hratch M Baghdassarian, Alisa N Truong, Peter W Kim, Anup K Singh, Nathan J Hillson, Jay D Keasling. De novo DNA synthesis using polymerase-nucleotide conjugates. Nat Biotechnol 36, 645–650 (2018).
  2. Kai Deng, Jijiao Zeng, Gang Cheng, Jian Gao, Kenneth L Sale, Blake A Simmons, Anup K Singh, Paul D Adams, Trent R Northen. Rapid characterization of the activities of lignin-modifying enzymes based on nanostructure-initiator mass spectrometry (NIMS). Biotechnol Biofuels 11, 266 (2018).
  3. Christopher R. Phaneuf, Betty Mangadu, Huu M. Tran, Yooli K. Light, Anchal Sinha, Frank W. Charbonier, Tyler P. Eckles, Anup K. Singh, Chung-Yan Koh, Integrated LAMP and immunoassay platform for diarrheal disease detection. Biosensors and Bioelectronics, Volume 120, 93-101, 2018.
  4. Todd A. Duncombe, Markus De Raad, Benjamin P. Bowen, Anup K. Singh, and Trent R. Northern, Insulator Nanostructure Desorption Ionization Mass Spectrometry. Analytical Chemistry 90 (16), 9657-9661, 2018.
  5. Brooke Harmon, Lily A. Chylek, Yanli Liu, Eshan D. Mitra, Avanika Mahajan, Edwin A. Saada, Benjamin R. Schudel, David A. Holowka, Barbara A. Baird, Bridget S. Wilson, William S. Hlavacek and Anup K. Singh. Timescale Separation of Positive and Negative Signaling Creates History-Dependent Responses to IgE Receptor Stimulation. Scientific reports 7 (1), 1-14, 2018.
  6. Kosuke Iwai, David Ando, Peter W Kim, Phillip C Gach, Manasi Raje, Todd A Duncomb, Joshua V Heinemann, Trent R Northen, Hector Garcia Martin, Nathan J Hillson, Paul D Adams, Anup K Singh. Automated flow-based/digital microfluidic platform integrated with onsite electroporation process for multiplex genetic engineering applications," IEEE Micro Electro Mechanical Systems (MEMS), pp. 1229-1232, 2018.
  7. C Gach, Philip, Iwai, Kosuke, Kim, Peter, Hillson, Nathan, & Singh, Anup. Droplet Microfluidics for Synthetic Biology, Lab Chip,17, 3388-3400, 2017.
  8. Brito, I. L., S. Yilmaz, K. Huang, L. Xu, S. D. Jupiter, A. P. Jenkins, W. Naisilisili, M. Tamminen, C. S. Smillie, J. R. Wortman, B. W. Birren, R. J. Xavier, P. C. Blainey, A. K. Singh, D. Gevers and E. J. Alm. "Mobile Genes in the Human Microbiome Are Structured from Global to Individual Scales." Nature 535, no. 7612 (2016): 435-+.
  9. Philip Charles Gach, Steve C.C. Shih, Jess Sustarich, Jay D Keasling, Nathan J Hillson, Paul D. Adams, and Anup K Singh. (2016) A Droplet Microfluidic Platform for Automating Genetic Engineering. ACS Synthetic Biology, 2016. DOI:10.1021/acssynbio.6b00011
  10. Steve C. C. Shih, Garima Goyal, Peter W. Kim, Nicolas Koutsoubelis, Jay D. Keasling, Paul D. Adams, Nathan J. Hillson, and Anup K. Singh, Versatile Microfluidic Device for Automating Synthetic Biology, ACS Synthetic Biology, 2016.
  11. Phaneuf, C. R., B. Mangadu, M. E. Piccini, A. K. Singh and C. Y. Koh. "Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices." Biosensors (Basel) 6, no. 4 (2016).
  12. Litvinov J, Moen ST, Koh CY, Singh AK. Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water. Biomicrofluidics. 2016 Jan 12;10(1):014103.
  13. Scott T. Moen, Christopher L. Hatcher, Anup K. Singh Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections, Plos One, 2016, 11(4): e0153137.
  14. Chung-Yan Koh, Ulrich Y. Schaff, Matthew E. Piccini, Larry H. Stanker, Luisa W. Cheng, Easwaran Ravichandran, Bal-Ram Singh, Greg J. Sommer, and Anup K. Singh. Centrifugal Microfluidic Platform for Ultrasensitive Detection of Botulinum Toxin, Anal Chem, 2015, 922-928.
  15. Shih, S.C.C., Gach, P.C., Sustarich, J., Adams, P.D., Singh, A.K., A droplet-to-digital (D2D) microfluidic device for screening single cells. Lab Chip. 2015,15(1):225-36.
  16. Rhee M, Light YK, Yilmaz S, Adams PD, Saxena D, Meagher RJ, Singh AK. Pressure stabilizer for reproducible picoinjection in droplet microfluidic systems. Lab Chip. 2014 Dec 7;14(23):4533-9.
  17. Rhee M, Liu P, Meagher RJ, Light YK, Singh AK. Versatile on-demand droplet generation for controlled encapsulation. Biomicrofluidics. 2014 Jun 12;8(3):034112.
  18. Chandrasekaran A, Singh AK. One-Pot, Microscale Cell-Free Enzyme Expression and Screening, Methods in Molecular Biology, 2014.
  19. Chang C, Sustarich J, Bharadwaj, R., Adams PD, Singh AK, Droplet-based microfluidic platform for heterogeneous enzymatic assays, Lab Chip, 2013, 13(9):1817-22.
  20. (Cover article) Aarthi Chandrasekaran, Kai Deng, Chung-Yan Koh, Taichi Takasuka, Lai F. Bergeman, Brian G. Fox, Paul D. Adams, and Singh AK, A Universal Flow Cytometry Assay for Screening Carbohydrate-Active Enzymes Using Glycan Microspheres, Chem. Commun., 2013,49, 5441-5443.
  21. Liu Y, Barua D, Zwartz G, Liu P, Wilson BS, Oliver JM, Hlavacek WS and Singh AK, Single-cell measurements of IgE-mediated FcεRI signaling using an integrated microfluidic platform, PLoS ONE 2013, 8(3): e60159.
  22. Wu M, Piccini M, Koh CY, Lam KS, Singh AK, Single cell microRNA analysis using microfluidic flow cytometry, PLoS ONE, 2013, 8(1): e55044.
  23. Schudel BR, Harmon B, Pruitt BW, Abhyankar VV, Negrete OA, Singh, AK Microfluidic platform for RNA interference screening of virus-host interactions, Lab Chip, 2013,13, 811-817.
  24. Wu M, Perroud TD, Srivastava N, Branda CS, Sale KL, Carson BD, Patel KD, Branda SS, Singh AK. Microfluidically-unified cell culture, sample preparation, imaging and flow cytometry for measurement of cell signaling pathways with single cell resolution. Lab Chip. 2012, 12(16):2823-31.
  25. (Cover article) Wu, M. and Singh AK, Single cell protein analysis, Current Opinion in Biotechnology, 2012, 23(1):83-8.
  26. Yilmaz, S and Singh AK, Single cell genome sequencing, Current Opinion in Biotechnology, 2012, 23(3):437-43.
  27. Kai Deng, Kevin W. George, Wolfgang Reindl, Jay D. Keasling, Paul D. Adams, Taek Soon Lee, Anup K. Singh, Trent R. Northen, Encoding substrates with mass tags to resolve stereospecific reactions using Nimzyme, Rapid Communications of Mass Spectrometry, 2011.
  28. Wolfgang Reindl, Cheng S, Kai Deng, Anup K. Singh, Blake A. Simmons, Paul D. Adams, and Trent R. Northen "Nanostructure-Initiator Mass Spectrometry (NIMS) for the Analysis of Enzyme Activities", Current Opinions in Chemical Biology, 2011.
  29. (Cover Article) Peng Liu, Robert J. Meagher, Yooli Kim Light, Suzan Yilmax, Romy Chakraborty, Adam P. Arkin, Terry C. Hazen and Anup K. Singh. Microfluidic fluorescence in situ hybridization and flow cytometry (mFlowFISH), Lab On a Chip, 11(16) :2673-9, 2011.
  30. Chhabra SR, Joachimiak MP, Petzold CJ, Zane GM, Price MN, Reveco SA, Fok V, Johanson AR, Batth TS, Singer M, Chandonia JM, Joyner D, Hazen TC, Arkin AP, Wall JD, Singh AK, Keasling JD. Towards a Rigorous Network of Protein-Protein Interactions of the Model Sulfate Reducer Desulfovibrio vulgaris Hildenborough. PLoS ONE 6(6), 2011.
  31. Wolfgang Reindl, Kai Deng, John M. Gladden, Gang Cheng, April Wong, Steven W. Singer, Seema Singh, Jinq-Chyi Lee, Chun-Hsu Yao, Terry C. Hazen, Anup K. Singh, Blake A. Simmons, Paul D. Adams, and Trent R. Northen. Colloid-based multiplexed screening for plant biomass-degrading glycoside hydrolase activities in microbial communities, Energy & Environmental Science, 2011. 4(8): p. 2884-2893.
  32. Sommer, G. J.; Mai J, Singh, A. K.; Hatch, A. V. Microscale Isoelectric Fractionation Using Photopolymerized Membranes, Analytical Chemistry 2011 83 (8), pp 3186–3191.
  33. Smruti Pushalkar, Shrinivasrao P. Mane, Xiaojie Ji, Yihong Li, Clive Evans, Oswald R. Crasta,, Douglas Morse, Robert Meagher, Anup Singh & Deepak Saxena, Microbial diversity in saliva of oral squamous cell carcinoma. FEMS Immunol Med Microbiol, (2011) 1–9
  34. J.S. Kinney, T. Morelli, T. Braun, C.A. Ramseier, A.E. Herr, J.V. Sugai, C.E. Shelburne, L.A. Rayburn, A.K. Singh, and W.V. Giannobile, Saliva and Pathogen biomarker Signatures and Periodontal Disease Progression. J Dent Res. 90(6):752-8, 2011.
  35. Chandrasekaran A, Bharadwaj R, Park JI, Sapra R, Adams PD, Singh AK. A microscale platform for integrated cell-free expression and activity screening of cellulases. J Proteome Res. 2010 Nov 5;9(11):5677-83.
  36. Bharadwaj, R., A. Wong, B. Knierim, S. Singh, B.M. Holmes, M. Auer, B.A. Simmons, P.D. Adams, A.K. Singh, "High-Throughput Enzymatic Hydrolysis of Lignocellulosic Biomass via In-Situ Regeneration," Bioresource Technology, 2010.
  37. (Cover Article) Bharadwaj, R., Z. Chen, S. Datta, B.M. Holmes, R. Sapra, B.A. Simmons, P.D. Adams, A.K. Singh, "Microfluidic Glycosyl Hydrolase Screening for Biomass-to-Biofuels Conversion," Analytical Chemistry, 82 (22), 9135-9592, 2010.
  38. Hecht AH, Sommer GJ, Durland RH, Yang X, Singh AK, Hatch AV, "Aptamers as Affinity Reagents in an Integrated Electrophoretic Lab-on-a-Chip Platform", Anal Chem, 2010.
  39. Achyuthan KE, Adams PD, Datta S, Simmons BA, Singh AK, "Hitherto unrecognized fluorescence properties of coniferyl alcohol", Molecules. 2010 Mar 11;15(3):1645-67.
  40. James CD, Moorman MW, Carson BD, Branda CS, Lantz JW, Manginell RP, Martino A, Singh AK, "Nuclear translocation kinetics of NF-kappaB in macrophages challenged with pathogens in a microfluidic platform", Biomed Microdevices. 2009,11(3):693-700.
  41. Achyuthan KE, Adams PD, Simmons BA, Singh AK, "Spectroscopic analyses of the biofuels-critical phytochemical coniferyl alcohol and its enzyme-catalyzed oxidation products" Molecules. 2009 23;14(11):4758-78
  42. K.E. Achyuthan, P.D. Adams, B.A. Simmons & A. K. Singh, "Multimode Spectroscopic High-throughput Screening of Unmodified Coniferyl Alcohol and its Laccase-catalyzed Oxidation Products", Molecules, 2009, 14(11):4758-78.
  43. G. J. Sommer, A. K. Singh and A. V. Hatch Enrichment and fractionation of proteins via microscale pore limit electrophoresis. Lab on a Chip, June 2009.
  44. N. Srivastava, J. S. Brennan, R. F. Renzi, M. Wu, S. S. Branda, A. K. Singh, and A. E. Herr Fully Integrated Microfluidic Platform Enabling Automated Phosphoprofiling of Macrophage Response. Anal. Chem., 81, 3261–3269, 2009.
  45. C.A. Ramseier, J.S. Kinney, A.E. Herr, T. Braun, J.V. Sugai, C.A. Shelburne, L.A. Rayburn, H.M. Tran, A.K. Singh, W.V. Giannobile, "Identification of pathogen and host-response markers correlated with periodontal disease", J Periodontol, 80, 3, 436-446, 2009.
  46. Perroud, TD, RJ Meagher, MP Kanouff, RF Renzi, M Wu, AK Singh, KD Patel, Isotropically etched radial micropore for cell concentration, immobilization, and picodroplet generation. Lab on a Chip, 9(4):507-15, 2009.
  47. (Cover article) R. J. Meagher, A. V. Hatch, R. F. Renzi, and A. K. Singh, "An Integrated, Portable Platform for Ultrasensitive and Rapid Detection of Biological Toxins". Lab on a Chip, 8, 2046–2053, 2008.
  48. (Featured on back cover) C. T. Lo, D. J. Throckmorton, A. K. Singh & A. E. Herr. "Photopolymerized Diffusion-Defined Polyacrylamide Gradient Gels for On-chip Protein Sizing." Lab Chip, 2008, 8, 1273-1279
  49. (Cover article) R. J. Meagher, Y. K. Light and A. K. Singh, "Rapid, continuous purification of proteins in a microfluidic device using genetically-engineered partition tags", Lab On A Chip, 2008.
  50. Reichmuth DS, Wang SK, Barrett LM, Throckmorton DJ, Einfeld W, Singh AK, "Rapid microchip-based electrophoretic immunoassays for the detection of swine influenza virus", Lab Chip. 2008, 8(8):1319-24.
  51. G. Sommer; A. K. Singh; A. V. Hatch "On-chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients", Analytical Chemistry, 2008, 80: 3327-33.
  52. S. Gaucher, A. Redding, A.M. Mukhopadhyay, J.D. Keasling, A.K. Singh, "Proteins in the Sulfate Reduction Pathway of Desulfovibrio vulgaris Hildenborough are Highly Modified", Journal of Proteome Research, 7, 2320-2331, 2008.
  53. Perroud TD, Kaiser JN, Sy JC, Lane TW, Branda CS, Singh AK, Patel KD, "Microfluidic-based cell sorting of Francisella tularensis infected macrophages using optical forces", Anal Chem. 2008, 80(16):6365-72.
  54. Meagher, RJ, AK Singh. (2008). Microchips containing in situ patterned polymeric media for biochemical analysis. In Biological Applications of Microfluidics, FA Gomez, Ed. Wiley and Sons: Hoboken, NJ, pp. 409–432.
  55. A.E. Herr, A. V. Hatch, D.J. Throckmorton, H.M. Tran, J.S. Brennan, W. V. Giannobile, A.K. Singh. "A Rapid Bioassay for Endogenous Matrix Metalloproteinase-8 in Saliva", Proceedings of the National Academy of Sciences of the USA, 2007, 104: 5268–5273.
  56. A.E. Herr, A. V. Hatch, D.J. Throckmorton, H.M. Tran, J.S. Brennan, W. V. Giannobile, A.K. Singh."Integrated Microfluidic Platform for Oral Diagnostics", Annals of NY Academy of Sciences, 2007, 1098: 362–374.
  57. R. Sapra , S.P. Gaucher, J.S. Lachmann, G.M. Buffleben , G. S. Chirica , J.E. Comer, J.W. Peterson, and A.K. Chopra and A.K. Singh, Proteomic analyses of murine macrophages treated with Bacillus anthracis lethal toxin Microbial Pathogenesis, 2006.
  58. AV Hatch, AE Herr, DJ Throckmorton, JS Brennan, and AK Singh. "Integrated Preconcentration SDS-PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels." Analytical Chemistry, 2006, 78(14), 4976 – 4984.
  59. S.R. Chhabra, Q. He, K.H. Huang, S.P. Gaucher, E.J. Alm, Z. He, M.Z. Hadi, T.C. Hazen, J.D. Wall, J. Zhou, A.P. Arkin, A.K. Singh, Global Analysis of Heat Shock Response in Desulfovibrio vulgaris Hildenborough Journal of Bacteriology. Mar. 2006; Vol. 188, No. 5, p. 1817–1828.
  60. Song, S; Singh, AK On-chip sample preconcentration for integrated microfluidic analysis, Anal Bioanal Chem; 2006; v.384, no.1, p.41-43.
  61. Barrett, L.M.; Skulan, A.J.; Singh, A.K.; Cummings, E.B.; Fiechtner, G.J. Dielectrophoretic Manipulation of Particles and Cells Using Insulating Ridges in Faceted Prism Microchannels Analytical Chemistry, 2005.
  62. Skulan, A.J.; Barrett, L.M.; Singh, A.K.; Cummings, E.B.; Fiechtner, G.J. Fabrication and Analysis of Spatially Uniform Field Electrokinetic Flow Devices: Theory and Experiment Anal. Chem. 2005.
  63. Moran-Mirabal, J.M; Edel, J.B.; Meyer, G.D.; Throckmorton, D.J.; Singh, A.K.; Craighead, H.G. "Micrometer-Sized Supported Lipid Bilayer Arrays for Bacterial Toxin Binding Studies through Total Internal Reflection Fluorescence Microscopy", Biophysical Journal, 2005.
  64. Herr, A.E.; Throckmorton, D.J.; Davenport, A.A.; Singh, A.K. "On-chip native gel electrophoresis-based immunoassays for tetanus antibody and toxin," Analytical Chemistry, Jan 15 2005; 77(2), 585-590
  65. Yu, D; Volponi, J; Chhabra, S; Brinker, CJ; Mulchandani, A; Singh, AK "Aqueous sol-gel encapsulation of genetically engineered Moraxella spp. cells for the detection of organophosphates", Biosensors & Bioelectronics; 15 Jan. 2005; vol.20, no.7, p.1433-7
  66. Schulte, S.; Singh, A.K.; Rauk, E.; Palmer, C.P. "Performance and selectivity of polymeric pseudostationary phases for the electrokinetic separation of amino acid derivatives" Anal Bioanal Chem (2005)382:777-782.
  67. Herr, A.E.; Singh, A.K. "Photopolymerized cross-linked polyacrylamide gels for on-chip protein sizing," Analytical Chemistry, Aug 15 2004; 76(16), 4727-4733
  68. Han, J; Singh, AK "Rapid protein separations in ultra-short microchannels: Microchip sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing" Journal of Chromatography A; Sep 17 2004; v.1049, no.1-2, p.205-209
  69. Pathak, S; Singh, AK; McElhanon, JR; Dentinger, PM. Dendrimer-activated surfaces for high density and high activity protein chip applications, Langmuir; Jul 20 2004; v.20, no.15, p.6075-6079
  70. Song, S; Singh, AK; Kirby, BJ "Electrophoretic concentration of proteins at laser-patterned nanoporous membranes in microchips ", Analytical Chemistry; Aug 1 2004; v.76, no.15, p.4589-4592
  71. Song, Y.J.; Yang, Y.; Medforth, C.; Pereira, E.; Singh, A.K.; Xu, H. Jiang, Y. Brinker, C. J.; van Swol, F.; Shelnutt, J. A. "Controlled Synthesis of 2-D and 3-D Platinum Dendrites Using Porphyrin Photocatalysts, Journal of the American Chemical Society, 126(2), 635-645, 2004.
  72. Song, S.; Singh, A.K.; Shepodd, T.J.; Kirby, B.J. "Microchip Dialysis of Proteins Using in Situ Photopatterned Nanoporous Polymer Membranes" Analytical Chemistry; (2004) 76(8) p.2367-2373
  73. Fintschenko Y, Kirby BJ, Hasselbrink, Jr. EF, Singh AK, Shepodd TJ, "Monolithic Materials: Miniature and Microchip Technologies," in Monolithic Materials: Preparation, Properties, and Applications Elsevier, Amsterdam (2003)
  74. Cummings, E.B.; Singh, A.K., "Dielectrophoresis in Microchips Containing Arrays of Insulating Posts: Theoretical and Experimental Results", Analytical Chemistry, 75(18), 4724-4731, 2003.
  75. Wang, K.; Singh, A.K, van Zanten, J.H.; "Aggregation Rate Measurements by Zero Angle Time-Resolved Multiangle Laser Light Scattering", Langmuir, 18(6); 2421-2425, 2002.
  76. (Cover Article) Throckmorton, D.J.; Shepodd, T.J.; Singh, A.K. "Electrochromatography in Microchips: Reversed-phase Separation of Peptides and Amino Acids Using Photo-Patterned Rigid Polymer Monoliths", Analytical Chemistry, 74, 784-789, 2002.
  77. Shediac, R.; Ngola, S.M.; Throckmorton, D.J.; Anex, D.S.; Shepodd, T.J.; Singh, A.K. "Reverse-Phase Electrochromatography of Amino Acids and Peptides Using Porous Polymer Monoliths", Journal of Chromatography A, 925, 251-262, 2001.
  78. Alfonta, L.; Willner, I; Throckmorton, D.J.; Singh, A.K., "Electrochemical and Quartz-Crystal-Microbalance Detection of the Cholera Toxin Employing Horseradish Peroxidase- and GM1-functionalized Liposomes", Analytical Chemistry, 73, 5287-5295, 2001.
  79. Singh, A.K.; Cummings, E.B.; Throckmorton, D.J. "Fluorescent Liposome Flow Markers for Microscale Particle-Image Velocimetry", Analytical Chemistry, 73, 1057-1061, 2001.
  80. Alfonta, L.; Singh, A.K.; Willner, I. "Liposomes Labeled with Biotin and Horseradish Peroxidase: A Probe for the Enhanced Amplification of Antigen-Antibody or Oligonucleotide-DNA Sensing Processes by the Precipitation of an Insoluble Product on Electrodes", Analytical Chemistry, 73, 91-102, 2001
  81. Singh, A.K.; Harrison, S.H., Schoeniger, J.S. "Gangliosides as Receptors for Biological Toxins: Development of Sensitive Fluoroimmunoassays Using Ganglioside-Bearing Liposomes", Analytical Chemistry, 72, 6019-6024, 2000.
  82. Bhatia, R. B.; Brinker, C.J.; Gupta, A. K.; Singh, A.K. "An Aqueous Sol-Gel Process for Protein Encapsulation", Chemistry of Materials, 12, 2434-2441, 2000.
  83. Lightstone, F.C.; Prieto, M.C.; Singh, A.K.; Piqueras, M.C.; Whittal, R.M.; Knapp, M.S.; Balhorn, R.; Roe, D.C. "The Identification of Novel Small Molecule Ligands that Bind to Tetanus Toxin", Chemical Research in Toxicology, 13, 356-362, 2000.
  84. Singh AK, Flounders AW, Volponi JV, Ashley CS, Wally K, Schoeniger JS, "Development of sensors for direct detection of organophosphates. Part I: Immobilization, characterization and stabilization of acetylcholinesterase and organophosphate hydrolase on silica supports", Biosens Bioelectron. 1999, 14(8-9):703-13.
  85. Flounders AW, Singh AK, Volponi JV, Carichner SC, Wally K, Simonian AS, Wild JR, Schoeniger JS, "Development of sensors for direct detection of organophosphates.: Part II: sol-gel modified field effect transistor with immobilized organophosphate hydrolase". Biosens Bioelectron. 1999, 14(8-9):715-22.
  86. Singh, AK, JS Schoeniger, RG Carbonell. (1999). Liposomes as signal-enhancement agents in immunodiagnostic applications. In Biosensors and Their Applications, VC Yang, TT Ngo, Eds., Kluwer Academic/Plenum Publishing: New York, pp. 131–145.
  87. Singh AK, Kilpatrick PK, Carbonell RG, "Application of Antibody and Fluorophore-Derivatized Liposomes to Heterogeneous Immunoassays for D-dimer", Biotechnol Prog. 1996;12(2):272-80.
  88. Singh AK, Kilpatrick PK, Carbonell RG, "Noncompetitive immunoassays using bifunctional unilamellar vesicles or liposomes", Biotechnol Prog. 1995 11(3):333-41.
  89. Jones MA, Singh AK, Kilpatrick PK, Carbonell RG, "Preparation and Characterization of Ligand-Modified Labelled Liposomes for Solid Phase Immunoassays", J Lip Research, 1993, Vol. 3, No. 3: 793-804.