Principal Investigator and Research Engineering Staff

Center for Bioengineering
Center for Engineered Materials and Manufacturing (CEMM)
Center for Micro and Nanotechnology
Materials Engineering Division

Research Interests

Additive manufacturing and advanced materials development. Focus areas in volumetric additive manufacturing (VAM), photopolymer 3-D micromanufacturing techniques, advance photopolymer materials development, optical systems, micro-architected materials, biomaterials, tissue engineering, ex vivo culture systems.

Biological instrumentation, bioengineering, and biomedical microtechnology.

Prior work

1. Microfluidic approaches for the rapid separation, processing, analysis, and detection of biological targets.
2. Implantable neural electrodes for stimulation and recording with applications in investigative neuroscience as well as therapeutics and prosthetics.
3. Low-cost atomic force microscopy for undergraduate education.

Google Scholar profile

Ph.D. Electrical Engineering, Massachusetts Institute of Technology, 2017

M.S. Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 2003

B.S.E. Electrical Engineering, Princeton University, 2001

• D. M. Wirth, C. C. Li, J. K. Pokorski, H. K. Taylor, M. Shusteff, “Fundamental scaling relationships in additive manufacturing and their implications for future manufacturing and bio-manufacturing systems” Add Manuf, 84, 104081 (2024).
• E. Krumins, J. C. Lentz, B. Sutcliffe, A. Sohaib, P. L. Jacob, B. Brugnoli, VC Crucitti, R. Cavanagh, R. Owen, C. Moloney, L. Ruiz-Cantu, I. Francolini, S. M. Howdle, M. Shusteff, F. R. A. J. Rose, R. D. Wildman, Y. He, V. Taresco "Glycerol-based sustainably sourced resin for volumetric printing" Green Chemistry 26(3), 1345-1355 (2024).
• T. H. Weisgraber, M. P. De Beer, S. Huang, J. J. Karnes, C. C. Cook, M. Shusteff, “Virtual Volumetric Additive Manufacturing (VirtualVAM),” Adv Mater Technol, 8(23), 2301054 (2023).
• J. Madrid-Wolff, J. Toombs, R. Rizzo, et al “A review of materials used in tomographic volumetric additive manufacturing,” MRS Communications 13(5), 764-785 (2023).
• S. Huang, S. M. Adelmund, P. S. Pichumani, J. J. Schwartz, Y. Mengüç, M. Shusteff, T. J. Wallin,  “One-pot ternary sequential reactions for photopatterned gradient multimaterials” Matter 6(7), 2419-38 (2023).
• J. J. Karnes, T. H. Weisgraber, C. C. Cook, D. N. Wang, J. C. Crowhurst, C. A. Fox, B. S. Harris, J. S. Oakdale, R. Faller, M. Shusteff, "Isolating Chemical Reaction Mechanism as a Variable with Reactive Coarse-Grained Molecular Dynamics: Step-Growth versus Chain-Growth Polymerization" Macromolecules 56(6), 2225–33  (2023).
• D. S. Melchert, A. Tahmasebipour, X. Liu, J. Mancini, B. Moran, B. Giera, I. D Joshipura, M. Shusteff, C. D. Meinhart, C. L. Cobb, C. Spadaccini, D. S. Gianola, M. R. Begley, “Anisotropic Thermally Conductive Composites Enabled by Acoustophoresis and Stereolithography” Adv Func Mater 32(31), 2201687 (2022).
• C. M. Rackson, J. T. Toombs, M. P. De Beer, C. C. Cook, M. Shusteff, H. K. Taylor, R. R. McLeod, “Latent image volumetric additive manufacturing,” Opt Lett, 47(5), 1279-82 (2022).
• J. J. Schwartz, D. H. Porcincula, C. C. Cook, E. J. Fong, M. Shusteff, “Volumetric additive manufacturing of shape memory polymers,” Polym. Chem. 13, 1813-17 (2021).
• C. M. Rackson, K. M. Champley, J. T. Toombs. E. J. Fong, V. Bansal, H. K. Taylor, M. Shusteff, R. R. McLeod, “Object-space optimization of tomographic reconstructions for additive manufacturing,” Add Manuf 48A, 102367 (2021).
• C. C. Cook, E. J. Fong, J. J. Schwartz, D. H. Porcincula, A. C. Kaczmarek, J. S. Oakdale, B. D. Moran, K. M. Champley, C. M. Rackson, A. Muralidharan, R. R. McLeod, M. Shusteff, "Highly Tunable Thiol-Ene Photoresins for Volumetric Additive Manufacturing," Adv Mater 2003376 (2020).
• J. J. Karnes, T. H. Weisgraber, J. S. Oakdale, M. Mettry, M. Shusteff, J. Biener, "On the Network Topology of Crosslinked Acrylate Photopolymers: A Molecular Dynamics Case Study," J Phys Chem B, 124(41), 9204–15 (2020).
• B. E. Kelly, I. Bhattacharya, H. Heidari, M. Shusteff, C. M. Spadaccini, H. K. Taylor, "Volumetric additive manufacturing via tomographic reconstruction," Science 363(6431), 1075-1079 (2019).
• M. D. Porter, B. Giera, R. M. Panas, L. A. Shaw, M. Shusteff, and J. B. Hopkins, "Experimental characterization and modeling of optical tweezer particle handling dynamics," Applied Optics 57(22), 6565-71 (2018).
• M. Shusteff, A. E. M. Browar, B. E. Kelly, J. Henriksson, T. H. Weisgraber, R. M. Panas, N. X. Fang, C. M. Spadaccini, "One-step volumetric additive manufacturing of complex polymer structures," Sci. Advances Vol. 3, no. 12, eaao5496 (2017).
• B. Kelly, I. Bhattacharya, M. Shusteff, R. M. Panas, H. K. Taylor, C. M Spadaccini "Computed Axial Lithography (CAL): Toward Single Step 3D Printing of Arbitrary Geometries," arXiv:1705.05893 (2017).
• L. A. Shaw, S. Chizari, R. M. Panas, M. Shusteff, C. M. Spadaccini, and J. B. Hopkins, "Holographic optical assembly and photopolymerized joining of planar microspheres," Optics Letters 41(15), 3571-3574 (2016).
• M. Shusteff, J. K. Dudoff, A. E. M. Browar, L. A. Shaw, R. M. Panas, J. B. Hopkins, N. X. Fang, C. M. Spadaccini "Optimal Source Beam Shaping for Digital Holographic Lithography," in Proc.of the 2016 Imaging and Applied Optics Congress, Heidelberg, Germany, DW1D.2 (2016).
• M. Shusteff, R. M. Panas, J. Henriksson, B. E. Kelly, A. E. M. Browar, N. X. Fang, and C. M. Spadaccini, "Additive Fabrication of 3D Structures by Holographic Lithography," in Proceedings of the 27th Annual Solid Freeform Fabrication Symposium, Austin, TX, 1183-1192 (2016)
• N. B. Zuckerman, M. Shusteff, P. F. Pagoria and A. E. Gash "Microreactor Flow Synthesis of the Secondary High Explosive 2,6-Diamino-3,5-dinitropyrazine-1-oxide (LLM-105)," J. Flow Chemistry 5(3) 178-182 (2015).
• E. J. Fong, C. Huang, J. Hamilton, W. J. Benett, M. Bora, A. Burklund, T. R. Metz, M. Shusteff  "A Microfluidic Platform for Precision Small-Volume Sample Processing and its Use to Size Separate Biological Particles With an Acoustic Microdevice" J. Visualized Experiments 105, e53051 (2015).
• M. Bora and M. Shusteff, "coupling of acoustic modes in microfluidic channel devices" Lab on a Chip, 15(15), 3192-3202 (2015).
• S.-Y. Jung, T. Notton, E. J. Fong, M. Shusteff, and L. S. Weinberger, "Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting" Lab on a Chip, 15(4), 1000-1003 (2015).
• X. Zheng, H. Lee, T. H.Weisgraber, M. Shusteff, J. Deotte, E. B. Duoss, J. D. Kuntz, M. M. Biener, Q. Ge, J. A. Jackson, S. O. Kucheyev, N. X. Fang, C. M. Spadaccini, "Ultralight, Ultrastiff Mechanical Metamaterials" Science, 344(6190), 1373-1377 (2014).
• E. J. Fong, A. C. Johnston, T. Notton, S.-Y. Jung, K. A. Rose, L. S. Weinberger, M. Shusteff, "Acoustic Focusing With Engineered Node Locations for High-Performance Microfluidic Particle Separation" Analyst, 139(5), 1192-1200 (2014).
• M. M. Packard, E.K. Wheeler, E. C. Alocilja, M. Shusteff, "Performance evaluation of fast microfluidic thermal lysis of bacteria for diagnostic sample preparation" Diagnostics 3(1), 105-116 (2013).
• Invited presentation: "Ignoring the Detector: Fast, Modular, Agnostic Sample Preparation" The Knowledge Foundation’s  Integrating Sample Preparation Conference 2011, Dec 8-9, 2011 Washington, DC
• M. Shusteff, H.-Y. Cheng, C. Hoyle, P. H. Paul, D. W. Arnold "Real-time enzyme reaction monitoring and kinetics measurements on the expressLC platform," 14th Annual Conference of the Society for Biomolecular Sciences, June 6, 2008, St. Louis, MO.
• M. Shusteff, T. P. Burg, S. R. Manalis,  "Measuring Boltzmann's constant with a low-cost atomic force microscope: an undergraduate experiment,"  American Journal of Physics 74(10),  873-879 (2006).
• T. Atanasijevic, M. Shusteff, P. Fam, A. Jasanoff, "Calcium Sensors for Magnetic Resonance Imaging Based on Superparamagnetic Iron Oxide Nanoparticles and Calmodulin,"  Proceedings of the National Academy of Science USA 103(40), 14707-14712 (2006).

Patents

• US Patents #10,647,061 (2020) #11,370,173 (2022) System and Method for Computed Axial Lithography (CAL) for 3D Additive Manufacturing
• U.S. Patent #11,809,161 (2023) Computed Axial Lithography Optimization System
• U.S. Patent #11,639,031 (2023) Photocurable Resins for Volumetric Additive Manufacturing
• U.S. Patents #10,875,247 (2021), #11,701,827 (2023) Multi-Beam Resin Curing System and Method For Whole-Volume Additive Manufacturing
• U.S. Patent #11,072,160 (2021) System and Method For Stimulated Emission Depletion Projection Stereolithography
• U.S. Patent Application #15/651,861 (2016) Multi-beam resin curing system and method for whole-volume additive manufacturing
• US Patent #8,999,129 (2015) Liquid and Liquid-filled Porous Electrodes for Electrophoresis
• US Patent #8,727,129 (2014), #8,991,614 (2015), #9,321,050 (2016) Microfluidic Ultrasonic Particle Separators With Engineered Node Locations and Geometries
• US Patents #8,524,064 (2013), #8,900,434 (2014) Three Dimensional Microelectrode System for Dielectrophoresis

• R&D 100 Award 2013 (DNATrax)
• Popular Mechanics Breakthrough Innovation Award 2013 (Artificial Retina)
• R&D 100 Award 2009 (Artificial Retina)