University of California, Davis BS 06/1989 Chemistry
University of California, San Diego PhD 11/1995 Biophysics
1995-1997 Postdoc, Scripps Research Institute, La Jolla CA
1997-2000 Postdoc, Lawrence Livermore National Lab, Livermore CA
2000-2006 Staff Scientist, Lawrence Livermore National Lab, Livermore CA
2006-present Senior Staff Scientist, Lawrence Livermore National Lab, Livermore CA
Honors and Awards
1989 Graduate with Honors, UC Davis College of Letters and Science
2009 Outstanding Mentor Award, US DOE Office of Science
2012 Award for Excellence, Department of Homeland Security
My primary interest is basic research into molecular mechanism of bacterial infection. I am interested in experimental methods across multiple scales, from sub-molecular to multicellular length scales and millisecond to hours time scale, that can be combined to give us a more comprehensive understanding of the events orchestration occuring when a bacterial pathogen encounters its host.
I am a crystallographer by training and have used crystallography to interrogate the structure-function of virulence factors. I have also collaborated with microbiologists, microbial geneticist, genetic engineers, immunologists, biochemists, and microscopists as part of multidisciplinary teams working toward a multiscale understanding of host-pathogen interactions.
Zeng, Z.H., Castano, A.R., Segelke, B.W., Stura, E.A., Peterson, P.A. and Wilson, I.A., 1997. Crystal structure of mouse CD1: an MHC-like fold with a large hydrophobic binding groove. Science, 277(5324), pp.339-345.
Segelke, B.W., 2001. Efficiency analysis of sampling protocols used in protein crystallization screening. Journal of crystal growth, 232(1-4), pp.553-562.
Segelke, B.W., Schafer, J., Coleman, M.A., Lekin, T.P., Toppani, D., Skowronek, K.J., Kantardjieff, K.A. and Rupp, B., 2004. Laboratory scale structural genomics. Journal of structural and functional genomics, 5(1-2), pp.147-157.
Segelke, B., Knapp, M., Kadkhodayan, S., Balhorn, R. and Rupp, B., 2004. Crystal structure of Clostridium botulinum neurotoxin protease in a product-bound state: Evidence for noncanonical zinc protease activity. Proceedings of the National Academy of Sciences, 101(18), pp.6888-6893.
Feld, G.K., El-Etr, S., Corzett, M.H., Hunter, M.S., Belhocine, K., Monack, D.M., Frank, M., Segelke, B.W. and Rasley, A., 2014. Structure and function of REP34 implicates carboxypeptidase activity in Francisella tularensis host cell invasion. Journal of Biological Chemistry, 289(44), pp.30668-30679.
Franco, M., D'haeseleer, P.M., Branda, S.S., Liou, M.J., Haider, Y., Segelke, B.W. and El-Etr, S.H., 2018. Proteomic profiling of Burkholderia thailandensis during host infection using bio-orthogonal noncanonical amino acid tagging (BONCAT). Frontiers in cellular and infection microbiology, 8.