Brent W. Segelke


Portrait of  Brent W. Segelke
  • Title
    Bionanomaterials Group Leader
  • Email
  • Phone
    (925) 424-4752
  • Organization

Professional Experience

1995-1997       Postdoc, Scripps Research Institute, La Jolla CA

1997-2000       Postdoc, Lawrence Livermore National Lab, Livermore CA

2000-2006       Staff Scientist, Lawrence Livermore National Lab, Livermore CA

2006-2022       Senior Staff Scientist, Lawrence Livermore National Lab, Livermore CA

2022-Present   Bionanomaterials Group Leader

Research Interests

My primary interest is basic research into molecular mechanism of bacterial infection. I am interested in experimental methods across multiple scales, from sub-molecular to multicellular length scales and millisecond to hours time scale, that can be combined to give us a more comprehensive understanding of the event orchestration occurring when bacterial pathogens encounter and invade their host.

I am a crystallographer by training and have used crystallography to interrogate the structure-function of virulence factors. I have collaborated with microbiologists, microbial geneticists, genetic engineers, immunologists, biochemists, and microscopists as part of interdisciplinary teams working toward a multiscale understanding of pathogenesis.

University of California, San Diego, PhD, 11/1995, Biophysics

University of California, Davis, BS, 06/ 1989, Chemistry

Zeng, Z.H., Castano, A.R., Segelke, B.W., Stura, E.A., Peterson, P.A. and Wilson, I.A., 1997. Crystal structure of mouse CD1: an MHC-like fold with a large hydrophobic binding groove. Science, 277(5324), pp.339-345.

Segelke, B.W., 2001. Efficiency analysis of sampling protocols used in protein crystallization screening. Journal of crystal growth, 232(1-4), pp.553-562.

Segelke, B.W., Schafer, J., Coleman, M.A., Lekin, T.P., Toppani, D., Skowronek, K.J., Kantardjieff, K.A. and Rupp, B., 2004. Laboratory scale structural genomics. Journal of structural and functional genomics, 5(1-2), pp.147-157.

Segelke, B., Knapp, M., Kadkhodayan, S., Balhorn, R. and Rupp, B., 2004. Crystal structure of Clostridium botulinum neurotoxin protease in a product-bound state: Evidence for noncanonical zinc protease activity. Proceedings of the National Academy of Sciences, 101(18), pp.6888-6893.

Feld, G.K., El-Etr, S., Corzett, M.H., Hunter, M.S., Belhocine, K., Monack, D.M., Frank, M., Segelke, B.W. and Rasley, A., 2014. Structure and function of REP34 implicates carboxypeptidase activity in Francisella tularensis host cell invasion. Journal of Biological Chemistry, 289(44), pp.30668-30679.

Franco, M., D'haeseleer, P.M., Branda, S.S., Liou, M.J., Haider, Y., Segelke, B.W. and El-Etr, S.H., 2018. Proteomic profiling of Burkholderia thailandensis during host infection using bio-orthogonal noncanonical amino acid tagging (BONCAT). Frontiers in cellular and infection microbiology, 8.

Complete List of Published Work in Google Scholar (

1989              Graduate with Honors, UC Davis College of Letters and Science

2009              Outstanding Mentor Award, US DOE Office of Science

2012              Award for Excellence, Department of Homeland Security