Brent Segelke

Staff Scientist
Bioscience and Biotechnology Division
Phone: +19254244752


University of California, Davis             BS      06/1989  Chemistry

University of California, San Diego     PhD    11/1995  Biophysics


Professional Experience

1995-1997       Postdoc, Scripps Research Institute, La Jolla CA

1997-2000       Postdoc, Lawrence Livermore National Lab, Livermore CA

2000-2006       Staff Scientist, Lawrence Livermore National Lab, Livermore CA

2006-present   Senior Staff Scientist, Lawrence Livermore National Lab, Livermore CA


Honors and Awards

1989              Graduate with Honors, UC Davis College of Letters and Science

2009              Outstanding Mentor Award, US DOE Office of Science

2012              Award for Excellence, Department of Homeland Security


Research Interests

My primary interest is basic research into molecular mechanism of bacterial infection. I am interested in experimental methods across multiple scales, from sub-molecular to multicellular length scales and millisecond to hours time scale, that can be combined to give us a more comprehensive understanding of the events orchestration occuring when a bacterial pathogen encounters its host.

I am a crystallographer by training and have used crystallography to interrogate the structure-function of virulence factors. I have also collaborated with microbiologists, microbial geneticist, genetic engineers, immunologists, biochemists, and microscopists as part of multidisciplinary teams working toward a multiscale understanding of host-pathogen interactions.


Selected Publications

Zeng, Z.H., Castano, A.R., Segelke, B.W., Stura, E.A., Peterson, P.A. and Wilson, I.A., 1997. Crystal structure of mouse CD1: an MHC-like fold with a large hydrophobic binding groove. Science, 277(5324), pp.339-345.

Segelke, B.W., 2001. Efficiency analysis of sampling protocols used in protein crystallization screening. Journal of crystal growth, 232(1-4), pp.553-562.

Segelke, B.W., Schafer, J., Coleman, M.A., Lekin, T.P., Toppani, D., Skowronek, K.J., Kantardjieff, K.A. and Rupp, B., 2004. Laboratory scale structural genomics. Journal of structural and functional genomics, 5(1-2), pp.147-157.

Segelke, B., Knapp, M., Kadkhodayan, S., Balhorn, R. and Rupp, B., 2004. Crystal structure of Clostridium botulinum neurotoxin protease in a product-bound state: Evidence for noncanonical zinc protease activity. Proceedings of the National Academy of Sciences, 101(18), pp.6888-6893.

Feld, G.K., El-Etr, S., Corzett, M.H., Hunter, M.S., Belhocine, K., Monack, D.M., Frank, M., Segelke, B.W. and Rasley, A., 2014. Structure and function of REP34 implicates carboxypeptidase activity in Francisella tularensis host cell invasion. Journal of Biological Chemistry, 289(44), pp.30668-30679.

Franco, M., D'haeseleer, P.M., Branda, S.S., Liou, M.J., Haider, Y., Segelke, B.W. and El-Etr, S.H., 2018. Proteomic profiling of Burkholderia thailandensis during host infection using bio-orthogonal noncanonical amino acid tagging (BONCAT). Frontiers in cellular and infection microbiology, 8.

Complete List of Published Work in Google Scholar