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Title
Deputy Group Leader, Laser Material Interaction Science -
Email
laurence2@llnl.gov -
Phone
(925) 422-1788 -
Organization
Not Available
Research Interests
- Protein folding
- Protein-protein and protein-DNA interactions
- Single molecule detection and spectroscopy
- Surface-enhanced Raman spectroscopy
- Ultra-sensitive micro-spectroscopy of optical materials
Ph.D., Physics, U.C. Berkeley, 2002
B.S., Physics, Caltech, 1997
- A. Ingargiola, T. Laurence, R. Boutelle, S. Weiss, X. Michalet. "Photon-HDF5: An Open File Format for Timestamp-Based Single-Molecule Fluorescence Experiments." Biophysical Journal, 2016. 110 (1): 26-33.
- Baxamusa, S., T. Laurence, M. Worthington, P. Ehrmann. "Photo-oxidation of polymer-like amorphous hydrogenated carbon under visible light illumination." Polymer Degradation and Stability, 2015. 122: 133-138.
- Baxamusa, S. H., A. Suresh, P. Ehrmann, T. Laurence, J. Hanania, J. Hayes, S. Harley, D. D. Burkey. "Photo-oxidation of Polymers Synthesized by Plasma and Initiated CVD." Chemical Vapor Deposition, 2015. 21 (10-11-12): 267-274.
- Li, J., S. S. Ross, Y. Liu, Y. X. Liu, K. Wang, H. Chen, F. Liu, T. A. Laurence, G. Liu, "Engineered Nanostructures of Haptens Lead to Unexpected Formation of Membrane Nanotubes Connecting Rat Basophilic Leukemia Cells." ACS Nano, 2015. 9 (7): 6738–6746. (Selected for Cover of ACS Nano)
- Ly, S., T. A. Laurence, N. Shen, B. Hollingsworth, M. Norton, and J. D. Bude. "Gigashot Optical Degradation in Silica Optics at 351 nm." Optics Express, 2015. 23 (4): 4074.
- Lindl, J. D., O. L. Landen, J. Edwards, E. I. Moses, et al. "Review of the National Ignition Campaign 2009-2012." Physics of Plasmas, 2014. 21, 020501. Erratum: Physics of Plasmas, 2015. 21, 129902.
- Laurence, T. A., S. Ly, F. Bourguet, N. O. Fischer, and M. A. Coleman. "Fluorescence Correlation Spectroscopy at Micromolar Concentrations without Optical Nanoconfinement." The Journal of Physical Chemistry B, 2014. 118 (32): 9662–67. Selected for special ACS Photonics Issue on "Probing the Fundamentals of Light-Matter Interactions," which showcases a "diverse range of cutting-edge, light-based characterization and spectroscopy manuscripts that showcase how light continues to be at the forefront of some of the most exciting developments by ACS authors."
- Raman, R N, S Elhadj, T. A. Laurence, and M J Matthews. "The Role of Electronic Defects and Brittle Microstructure in Laser-Driven Material Failure." Journal of Physics D: Applied Physics, 2014. 47 (34): 345304.
- Laurence, T. A., J. D. Bude, N. Shen, W. A. Steele, and S. Ly. "Quasi-continuum Photoluminescence: Unusual Broad Spectral and Temporal Characteristics Found in Defective Surfaces of Silica and Other Materials." Journal of Applied Physics, 2014. 115 (8): 083501.
- Bude, J., P. Miller, S. Baxamusa, N. Shen, T. Laurence, W. Steele, T. Suratwala, et al. "High Fluence Laser Damage Precursors and Their Mitigation in Fused Silica." Optics Express, 2014. 22 (5): 5839.
- Ly, S., F. Bourguet, N. O. Fischer, E. Y. Lau, M. A. Coleman, and T. A. Laurence. 2014. "Quantifying Interactions of a Membrane Protein Embedded in a Lipid Nanodisc Using Fluorescence Correlation Spectroscopy." Biophysical Journal, 2014. 106 (2): L05–L08.
- Conway, A. M., L. F. Voss, A. J. Nelson, P. R. Beck, T. A. Laurence, R. T. Graff, R. J. Nikolic, S. A. Payne, H. Kim, L. J. Cirignano, and K. Shah. "Fabrication Methodology of Enhanced Stability Room Temperature TlBr Gamma Detectors." IEEE Transactions on Nuclear Science, 2013. 60 (2): 1231–1236.
- Ly, S., R. Altman, J. Petrlova, Y. Lin, S. Hilt, T. Huser, T. A. Laurence, and J. C. Voss. 2013. "Binding of Apolipoprotein E Inhibits the Oligomer Growth of Amyloid-beta Peptide in Solution as Determined by Fluorescence Cross-correlation Spectroscopy." Journal of Biological Chemistry, 2013. 288 (17): 11628–11635.
- Dertinger, T., A. Pallaoro, G. Braun, S. Ly, T. A. Laurence, and S. Weiss, "Advances in Superresolution Optical Fluctuation Imaging (SOFI)." Quarterly Reviews of Biophysics, 2013. 46 (2): 210-221.
- Shen, N., P.E. Miller, J.D. Bude, T.A. Laurence, T.I. Suratwala, W.A. Steele, M.D. Feit, and L.L. Wong. "Thermal annealing of laser damage precursors on fused silica surfaces." Optical Engineering, 2012. 51 (12). doi: 10.1117/1.OE.51.12.121817.
- Cho, H., E.-C. Yeh, R. Sinha, T. A. Laurence, J. P. Bearinger, and L. P. Lee, "Single-Step Nanoplasmonic VEGF165 Aptasensor for Early Cancer Diagnosis." ACS Nano, 2012. 6 (9): 7607–7614.
- Laurence, T.A., G.B. Braun, N.O. Reich, and M. Moskovits, "Robust SERS Enhancement Factor Statistics Using Rotational Correlation Spectroscopy." Nano Letters, 2012. 12(6): 2912-2917.
- Laurence, T.A., J.D. Bude, S. Ly, N. Shen, M.D. Feit, "Extracting the distribution of laser damage precursors on fused silica surfaces for 351 nm, 3 ns laser pulses at high fluences (20-150 J/cm2)." Optics Express, 2012. 20(10): 11561-11573.
- Yim, S.W., T. Kim, T.A. Laurence, S. Partono, D. Kim, Y. Kim, S. Weiss, and A. Reitmair, "Four-Color Alternating-Laser Excitation Single-Molecule Fluorescence Spectroscopy for Next-Generation Biodetection Assays." Clinical Chemistry, 2012. 58: 707-716.
- Ly, S., J. Petrlova, T. Huser, S. Fore, T. Gao, J. Voss, and T.A. Laurence, "Stoichiometry of Reconstituted High-Density Lipoproteins in the Hydrated State Determined by Photon Antibunching." Biophysical journal, 2011. 101(4): p. 970.
- Laurence, T.A., N. Shen, J.D. Bude, "Ultra-fast photoluminescence in fused silica surface flaws susceptible to laser damage." MRS Online Proceedings Library, 2011. 1365: mrss11-1365-tt08-04.
- P.J. Pauzauskie, J.C. Crowhurst, M.A. Worsley, T.A. Laurence, A.L.D. Kilcoyne, Y. Wang, T.M. Willey, K.S. Visbeck, S.C. Fakra, W.J. Evans, J.M. Zaug, and J.H. Satcher, Jr., "Synthesis and characterization of a nanocrystalline diamond aerogel." Proceedings of the National Academy of Sciences of the United States of America, 2011. 108(21): 8550–8553.
- T.J. Gao, C.D. Blanchette, W. He, F. Bourguet, S. Ly, F. Katzen, W.A. Kudlicki, P.T. Henderson, T.A. Laurence, T. Huser, and M.A. Coleman, "Characterizing diffusion dynamics of a membrane protein associated with nanolipoproteins using fluorescence correlation spectroscopy". Protein Science, 2011. 20(2): p. 437-447.
- T.I. Suratwala, P.E. Miller, J.D. Bude, W.A. Steele, N. Shen, M.V. Monticelli, M.D. Feit, T.A. Laurence, M.A. Norton, C.W. Carr, and L.L. Wong, "HF-Based Etching Processes for Improving Laser Damage Resistance of Fused Silica Optical Surfaces." J. Am. Ceram. Soc., 2011. 94(2): 416-428.
- Miller, P.E., J.D. Bude, T.I. Suratwala, N. Shen, T.A. Laurence, W.A. Steele, J. Menapace, M.D. Feit, and L.L. Wong, "Fracture-induced subbandgap absorption as a precursor to optical damage on fused silica surfaces." Opt. Lett., 2010. 35(16): p. 2702.
- Laurence, T.A., B. Chromy, "Efficient maximum likelihood estimator fitting of histograms." Nature Methods, 2010. 7:338-339.
- A.J. Nelson, T.A. Laurence, A.M. Conway, E.M. Behymer, B.W. Sturm, L.F. Voss, R.J. Nikolic, S.A. Payne, A. Mertiri, G. Pabst, K.C. Mandal, A. Burger, "Spectroscopic investigation of (NH4)2S treated GaSeTe for radiation detector applications." Materials Letters, 2010. 64:393–395.
- Laurence, T.A., J.D. Bude, N. Shen, T. Feldman, P.E. Miller, W.A. Steele, T. Suratwala, "Near surface metallic-like photoluminescence and absorption in fused silica." Applied Physics Letters, 2009. 94: 151114.
- Miller, A.E., C.W. Hollars, S.M. Lane, and T.A. Laurence, Fluorescence Cross-Correlation Spectroscopy as a Universal Method for Protein Detection with Low False Positives. Analytical Chemistry, 2009. 81(14): p. 5614.
- Braun, G.B., S.J. Lee, T.A. Laurence, N. Fera, L. Fabris, G.C. Bazan, M. Moskovits, and N.O. Reich, Generalized Approach to SERS-Active Nanomaterials via Controlled Nanoparticle Linking, Polymer Encapsulation, and Small-Molecule Infusion. The Journal of Physical Chemistry C, 2009. 113(31): p. 13622.
- Laurence, T.A., C. Talley, A. Schwartzberg, G. Braun, M. Moskovits, N. Reich, and T. Huser, "Rapid, solution-based characterization of optimized SERS nanoparticle substrates." Journal of the American Chemical Society, 2009. 131(1): 162–169.
- Cho, H., B.R. Baker, S. Wachsmann-Hogiu, C.V. Pagba, T.A. Laurence, S.M. Lane, L.P. Lee, and J.B.H. Tok, "Aptamer-based SERRS Sensor for Thrombin Detection." Nano-letters, 2008. 8(12): 4386-4390.
- Laurence, T. A., Y. Kwon, A. Johnson, C. W. Hollars, M. O'Donnell, J. A. Camarero and D. Barsky. "Motion of a DNA sliding clamp observed by single molecule fluorescence spectroscopy." J Biol Chem, 2008. 283(34): 22895-22906.
- Laurence, T.A., Y. Kwon, E. Yin, C.W. Hollars, J.A. Camarero, and D. Barsky, "Correlation Spectroscopy of Minor Fluorescent Species: Signal Purification and Distribution Analysis." Biophysical Journal, 2007. 92: 2184-2198.
- Fore, S., T.A. Laurence, C.W. Hollars, and T. Huser, "Counting constituents in molecular complexes by fluorescence photon antibunching". IEEE Journal of Selected Topics in Quantum Electronics, 2007. 13(4): 996-1005.
- Nir, E., X. Michalet, K.M. Hamadani, T.A. Laurence, D. Neuhauser, Y. Kovchegov, and S. Weiss, "Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments". J Phys Chem B, 2006. 110(44): p. 22103-24.
- Miller, A.E., A.J. Fischer, T.A. Laurence, C.W. Hollars, R.J. Saykally, J.C. Lagarias, and T. Huser, "Single molecule dynamics of phytochrome-bound fluorophores probed by fluorescence correlation spectroscopy". Proceedings of the National Academy of Sciences of the United States of America, 2006. 103(30): 11136–11141.
- Laurence, T.A., S. Fore, and T. Huser, "Fast, flexible algorithm for calculating photon correlations". Optics Letters, 2006. 31(6): 829-831.
- Laurence, T.A., X. Kong, M. Jaeger, and S. Weiss, "Probing structural heterogeneities and conformational fluctuations of biopolymers". Proceedings of the National Academy of Sciences of the United States of America, 2005. 102(48): 17348-17353.
- Kapanidis, A. N., E. Margeat, T.A. Laurence, S. Doose, S.O. Ho, J. Mukhopadhyay, E. Kortkhonjia, V. Mekler, R. H. Ebright, and S. Weiss, "Retention of transcription initiation factor σ70 in transcription elongation: single-molecule analysis". Molecular Cell, 2005. 20(3): 347-56.
- Fore, S., T.A. Laurence, Y. Yeh, R. Balhorn, C.W. Hollars, M. Cosman, T. Huser, "Distribution analysis of the photon correlation spectroscopy of discrete numbers of dye-molecules conjugated to DNA". IEEE Journal of Selected Topics in Quantum Electronics, 2005. 11(4): 873-880.
- Kapanidis, A. N., T.A. Laurence, N.K. Lee, E. Margeat, X. Kong, and S. Weiss, "Alternating-laser excitation of single biomolecules". Accounts of Chemical Research, 2005. 38(7): 523-533.
- Laurence, T.A., A.N. Kapanidis, X. Kong, D.S. Chemla, and S. Weiss, "Photon Arrival-Time Interval Distribution (PAID): A Novel Tool for Analyzing Molecular Interactions". Journal of Physical Chemistry B, 2004. 108: 3051-3067.
- Kapanidis, A. N., N. K. Lee, T. A. Laurence, S. Doose, E. Margeat and S. Weiss, "Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules". Proceedings of the National Academy of Sciences of the United States of America, 2004. 101(24): 8936-41.
- Michalet, X., A. N. Kapanidis, T. Laurence, F. Pinaud, S. Doose, M. Pflughoefft and S. Weiss, "The Power and Prospects of Fluorescence Microscopies and Spectroscopies". Annual Review of Biophysics and Biomolecular Structure, 2003. 32: 161-182.
- Deniz, A.A., T.A. Laurence, M. Dahan, D.S. Chemla, P.G. Schultz, and S. Weiss, "Ratiometric single-molecule studies of freely diffusing biomolecules". Annual Review of Physical Chemistry, 2001. 52: 233-253.
- Dahan, M., T.A. Laurence, F. Pinaud, D.S. Chemla, A.P. Alivisatos, M. Sauer, and S. Weiss, "Time-gated biological imaging by use of colloidal quantum dots". Optics Letters, 2001. 26: 825-827.
- Deniz, A.A., T.A. Laurence, G.S. Beligere, M. Dahan, A.B. Martin, D.S. Chemla, P.E. Dawson, P.G. Schultz, and S. Weiss, "Single-molecule protein folding: diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin Inhibitor 2". Proceedings of the National Academy of Sciences of the United States of America, 2000. 97: 5179-5184.
- Kern, B., T.A. Laurence, C. Martin, and P.E. Dimotakis, "Temporal coherence of individual turbulent patterns in atmospheric seeing". Applied Optics, 2000. 39: 4879-4885.
- Ha, T., T.A. Laurence, D.S. Chemla, and S. Weiss, "Polarization spectroscopy of single fluorescent molecules". Journal of Physical Chemistry B, 1999. 103: 6839-6850.