Nick Fischer

Staff Scientist
Bioscience and Biotechnology Division
Email: fischer29@llnl.gov
Phone: +19254226144

Education

Ph.D. Molecular and Cellular Biology (2005) University of Massachusetts – Amherst (Vincent Rotello)

B.S. Biology (1998) West Chester University of Pennsylvania

 

Professional Background

Staff Scientist (2008 – present), Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory

Postdoctoral Fellow (2005 – 2008), Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory (Jeffrey Tok)

Research Assistant (2000 – 2005), University of Massachusetts – Amherst, Molecular and Cellular Biology Program (Vincent Rotello)

 

Research Interests and Selected Publications

Biomimetic nanoparticle design for vaccine-related in vivo applications

  • Weilhammer D, Dunkle AD, Blanchette CD, Fischer NO, Corzett M, Lehmann D, Boone T, Hoeprich P, Driks A, Rasley A (2017) Enhancement of antigen-specific CD4+ and CD8+ T cell responses using a self-assembled biologic nanolipoprotein particle vaccine. Vaccine, 35, 1475-1481. PMID: 28214044.
  • Fischer NO, Rasley A, Corzett M, Hwang MH, Hoeprich PD, Blanchette CD (2013) Colocalized delivery of adjuvant and antigen using nanolipoprotein particles enhances the immune response to recombinant antigens. Journal of the American Chemical Society, 135, 2044-2047. PMID: 23331082.
  • Weilhammer DR, Blanchette CD, Fischer NO, Alam S, Loots GG, Corzett M, Thomas C, Lychak C, Dunkle AD, Ruitenberg JJ, Ghanekar SA, Sant AJ, and Rasley A (2013) Nanolipoprotein delivery enhances immunostimulatory properties of innate immune agonists and provides protection against lethal influenza challenge. Biomaterials, 34, 10305-10318. PMID: 24075406.

In vitro tissue mimetic platforms

  • Lam D, Enright HA, Cadena J, Peters SKG, Sales AP, Osburn J, Soscia DA, Kulp KS, Wheeler EK, and Fischer NO (2019) Tissue-specific extracellular matrix accelerates the formation of neural networks and communities in a neuron-glia co-culture on a multi-electrode array. Scientific Reports, 9, 4159. PMID: 30858401.
  • Belle AM, Enright HA, Sales AP, Kulp K, Osburn J, Kuhn EA, Fischer NO*, Wheeler EK* (2018) Evaluation of in vitro neuronal platforms as surrogates for in vivo whole brain systems. Scientific Reports, 8, 10820. (*Co-corresponding authors) PMID: 30018409.
  • Soscia DA, Belle AM, Fischer NO, Enright HA, Sales AP, Osburn J, Benett WJ, Mukerjee E, Kulp KS, Pannu S, Wheeler E (2017) Controlled placement of multiple CNS cell populations to create complex neuronal cultures. PLoS ONE, 12, e0188146. PMID: 29161298.

Biomimetic nanoparticle development and characterization

  • Anikeeva A, Fischer NO, Blanchette CD, Sykulev Y (2019) Extent of MHC clustering regulates selectivity and effectiveness of T-cell responses. Journal of Immunology, 202, 591-597. PMID: 30541879.
  • Gilmore SF, Carpenter TS, Ingolfsson HI, Peters SKG, Henderson PT, Blanchette CD, Fischer NO (2018) Lipid composition dictates rHDL serum stability: implications for in vivo applications. Nanoscale, 10, 7420-7430. PMID: 29564446.

Molecular recognition elements and detection platforms

  • Fischer NO, Tarasow TM, Tok JBH. (2008) Protein detection via direct enzymatic amplification of short DNA aptamers. Analytical Biochemistry, 373, 121-128. PMID: 17980857
  • Fischer NO, Tok JBH, Tarasow TM (2008) Massively parallel interrogation of aptamer sequence, structure and function. PloS ONE, 3, e2720. PMID: 18628955

Metal-core nanoparticles and applications

  • Fischer NO, Verma A, Goodman CM, Simard JM, Rotello VM (2003) Reversible "irreversible" inhibition of chymotrypsin using nanoparticle receptors. Journal of the American Chemical Society, 125, 13387-13391. (Profiled in Editor’s Choice, Science, 302, 536) PMID: 14583034
  • Fischer NO, McIntosh CM, Simard JM, Rotello VM (2002) Inhibition of chymotrypsin through surface binding using nanoparticle-based receptors. Proceedings of the National Academy of Sciences U.S.A., 99, 5018-5023. PMID: 11929986

Complete List of Published Work:   https://scholar.google.com/citations?user=9bHS5ZgAAAAJ&hl=en