I am a senior staff biomedical scientist at Lawrence Livermore National Laboratory in the division of Biology and Biotechnology. I am also adjunct professor in the department of Radiation Oncology at the University of California at Davis. I have authored over 120 publications in peer-reviewed journals, published proceedings and book chapters covering a diverse breadth of molecular biology and biochemistry. My major areas of research focus are in the area of radiobiology in support of cancer treatment and biodosimetry. My group has also been very active in the development of biotechnology and advance biochemical techniques around the areas of cell-free expression, membrane proteins and nanoparticles made of apolipoproteins called nanolipoprotein particles (NLPs).
ORCID ID: 0000-0003-1389-4018
RESEARCH AND PROFESSIONAL EXPERIENCE
2014 - presentAdjunct Professor, Department of Radiation Oncology, University of California, Davis. Health System.
2008 - presentSenior Staff Scientist, Physics and Life Sciences Directorate. Lawrence Livermore National Laboratory, Livermore, CA.
2008 - 2013 Associate Adjunct Professor, Department of Radiation Oncology, University of California, Davis. Health System.
2007- 2009 Junior Specialist, The NSF Center for Biophotonics, University of California, Davis. Health System.
2006 - 2008 Deputy Group Leader for Molecular Toxicology, Chemistry, Materials and Life Sciences Directorate. Lawrence Livermore National Laboratory, Livermore, CA.
2005 - 2006 Group leader for Intelligence Management, Biosciences, Lawrence Livermore National Laboratory, Livermore, CA.
2000 - 2005 Biomedical Senior Staff Scientist, Biology and Biotechnology Research Program. Lawrence Livermore National Laboratory, Livermore, CA.
1997 - 2000 Postdoctoral Fellow in the laboratory of Dr. Harvey Mohrenweiser, Lawrence Livermore National Laboratory, Livermore, CA.
1992 - 1996 Thesis research in the laboratory of Dr. Kenneth J. Rothschild, Boston University, Department of Physics, Boston, MA.
1990 - 1992 Research Assistant in the Laboratory of Dr. Robert Davenport, Boston University, Department of Chemistry, Boston, MA.
1987 - 1990 Senior Research Assistant for the Department of Chemistry, Boston University, Department of Chemistry, Boston, MA.
1983 - 1992 Commissioned Officer, U.S. Army Reserves. Various positions of fiscal and command responsibility.
LLNL, SI-LDRD. Wheeler (P.I.). Oct. 2018 – Sep. 2021.
The project will develop novel bioprinting approaches combined with engineered tools for characterizing cancer development and treatment.
NIH/NIAIDR21AI120925, Coleman (P.I.). July. 01, 2016 – Jun. 30, 2019
Synthetic Generation of a Chlamydia Vaccine.
The project will investigate the development of novel vaccines using synthetic biology to generate nanoparticles.
NIH/NCI R01CA172067. Dubios (P.I.). Aug. 2013 – Mar. 2018.
Optimizing 131I-miBG therapy for children with advanceneuroblastoma.
The central goal of this proposal is to demonstrate the combination of 131I-mIBG together with a systemic radiation sensitizer will improve upon the known single-agent activity of 131I-mIBG treatment.
NIH/NIGMS R01GM117342. Frank (P.I.). Mar. 2016 – Feb., 2021.
Technology development for biological imaging with x-ray free electron lasers.
The goal of this proposal was to develop techniques for imaging membrane proteins using XFELs at LCLS.
NIH/NCI R01CA213830 Li(P.I.). Feb. 2017 – Jan. 2022
Mitochondrial bioenergetics in aggressive breast cancer growth.
This project will develop novel nanoparticles for breast cancer stem cell detection and treatment.
NIH/NCI R01CA214912 Dubois (P.I.). Jan. 2018 – Dec. 2023
Tumor and host markers of clinical outcomes after MIBG therapy in neuroblastoma.
Project will look at treatment for primary tumors in patients with neuroblastoma.
TRISH-NASA. Chris Porada (P.I.). Nov. 2017 – Sep. 2021
Novel microfluidic biomarker detection platforms to monitor in vivo effects of solar particle events and galactic cosmic rays radiation, using mice with human hematopoietic systems.
NIH/NIAIDU19 Coleman/de la Maza (P.I.s). Jun. 2019 – May. 2024
Cooperative Research Center for NanoScaffold-Based Chlamydia trachomatis Vaccines.
This is a multidisciplinary project that will develop unique reagents for use in pathogen vaccine development.
NIH/NIAIDU01 Coleman (P.I.s). Dec. 2019 – Nov. 2024
LAMP-integrated detection for rapid and long term biodosimetry.
We will specifically focus on demonstrating utility of our BIO ID technology to discriminate between 0 and 2 Gy ionizing radiation exposures.
Postdoctoral Fellow, Livermore National Laboratory, Livermore, CA
Ph.D., Boston University, Boston, MA
B.S., University of Massachusetts, Boston, MA
Coleman, M.A., Cappuccio, J.A., Blanchette, C.D. Gao, T., Arroyo, E.S., Hinz, A.K., Bourguet, F.A., Segelkw, S., Hoeprich, P.D., Huser, T., Laurence, T., Motin, V.L. and Chromy, B.A. (2016) Expression and Association of the Yersinia pestis Translocon Proteins, YopB and YopD, Are Facilitated by Nanolipoprotein Particles. PLoS One. 11(3): e0150166. doi:10.1371
Edmondson, D.A., Karski, E.E., Kohlgruber, A., Koneru, H., Matthay, K.K. Allen, M.S., Peterson, L.E., DuBois, S.G. and Coleman, M.A. (2016) Transcript analysis for characterizing internal biodosimetry using peripheral blood from patients with neuroblastoma treated with 131I-mIBG, a targeted radionuclide. Rad Res. 186, 235-244. doi: 10.1667/RR14263.
Campbell, K., Karski, E.E., Olow, A., Edmondson, D.A., Kohlgruber, A.C., Coleman,M.A., Haas-Kogan, D.A, Matthay, K.K., and DuBois, S.G. (2017).Peripheral blood biomarkers associated with toxicity and treatment characteristics after 131I-MIBG therapy in patients with neuroblastoma. International Journal of Radiation Oncology Biology Physics. 99(2):468-475.
Trans, D.J., Bai, R., Addison, B., Liu, R., Hamel, E., Coleman, M.A. and Henderson P.T. (2017) Synthesis of two fluorescent GTPγS molecules and their biological relevance. Nucleosides, Nucleotides and Nucleic Acids.36(6):379-391. doi: 10.1080/15257770.
Yung, M.C., Bourguet, F.A., Carpenter, T.S., Coleman, M.A. (2017) Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage φX174 in Escherichia coli. Microbial Cell Factories. 12(6). PMID: 28586369.
Scharadin, T.M., He, W., Yiannakou, Y., Tomilov, A.A., Saldana, M., Cortopassi, G.A., Carraway III, K.A., Coleman, M.A. and Henderson, P.T. (2017) Synthesis and Biochemical Characterization of EGF Receptor in a Water-soluble Membrane Model System. PLoS One. 12(6): e0177761. https://doi.org/10.1371.
He, H., Felderman, M., Evans, A.C., Geng, J., Homan, D., Bourguet, F., Fischer, N.O., Li, Y., Lam, K.S., Noy, A., Xing, L., Cheng, R.H., Rasley, A., Blanchette, C.D., Kamrud, K., Wang, N., Gouvis, H., Peterson, T.C., Hubby, B. and Coleman, M.A. (2017) Cell-free production of a functional oligomeric form of a chlamydia major outer membrane protein (MOMP) for vaccine development. J. Biol. Chem.jbc.M117.784561. doi:10.1074/jbc.M117.784561.
Cleveland IV, T.E., He, W., Evans, A.C., Fischer, N., Lau, E.Y., Coleman, M.A. and Butler, P. (2018) SANS/SAXS demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Science.27(3);780-789.
Emilio Cordova, Macario Martínez-Castillo, PhD; Nicolas Villegas-Sepulveda; Marco Meraz-Rios; Araceli Hernandez-Zavala; Jaime Berumen; Matthew A Coleman; Lorena Orozco. (2018) Differential effects of Curcumin on Cell Cycle progression and Cell Death in acute and chronic myeloid leukemia derived cell lines. Oncol Lett. May;15(5):6777-6783. doi: 10.3892/ol.2018.8112.
Patriarchi, T., Shen, A., He, W., Baikoghli, M., Cheng, R.H., Xiang, Y.K., Coleman M.A. and Tian L. (2018) Nanodelivery of a functional membrane receptor to manipulate cellular phenotype. Scientific Reports 10.1038/s41598-018-21863-3.
Quinn, S.D., Srinivasan, S., Gordon, J.B., He, W., Carraway, K.L.III., Coleman, M.A. and Gabriela S. Schlau-Cohen, G.S. (2018)Single-Molecule Fluorescence Detection of the Epidermal Growth Factor Receptor in Membrane Discs.Biochemistry. DOI: 10.1021/acs.biochem.8b00089.
Seuring C, Ayyer K, Filippaki E, Barthelmess M, Longchamp JN, Ringler P, Pardini T, Wojtas DH, Coleman MA, Dörner K, Fuglerud S, Hammarin G, Habenstein B, Langkilde AE, Loquet A, Meents A, Riek R, Stahlberg H, Boutet S, Hunter MS, Koglin J, Liang M, Ginn HM, Millane RP, Frank M, Barty A, Chapman HN. (2018) Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. Nat Commun. 2018 May 9;9(1):1836. doi: 10.1038/s41467-018-04116-9.
Casadei CM, Tsai CJ, Barty A, Hunter MS, Zatsepin NA, Padeste C, Capitani G, Benner WH, Boutet S, Hau-Riege SP, Kupitz C, Messerschmidt M, Ogren JI, Pardini T, Rothschild KJ, Sala L, Segelke B, Williams GJ, Evans JE, Li XD, Coleman M, Pedrini B, Frank M. (2018) Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals. IUCrJ. 2018 Jan 1;5(Pt 1):103-117. doi: 10.1107/S2052252517017043. PMID: 29354276.
Wiedorn, M.O. et al., (2018) Megahertz serial crystallography. Nat Commun. 2018 9(1):4025. PMID: 30279492.
Gisriel, C, et al.,(2019) Membrane Protein Megahertz Crystallography at the European XFEL. Frontiers in Pharmacology. Nat Commun. Submitted.
Shelby, M.L. He, H. and Coleman, M.A. (2019) Cell-free co-translational approaches for producing mammalian receptors: Expanding the cell-free expression toolbox using nanolipoproteins. Frontiers in Pharmacology. Submitted.
2015 - presentUCD - Biophysics Graduate Group Professor/Member.
2010 - presentUCD - Pharmacology & Toxicology Graduate Group Professor/Member.
2018 - presentReviewer NIH SBIR PHS - Adjuvant Development for Vaccines and for Autoimmune and Allergic Diseases study section.
2018 LLNL 2018 Outstanding Mentor
2013 - 2015 Reviewer NIH Academic Research Enhancement Award (AREA) grants (R15)Oncology 2 – Translational Clinical IRG (OTC) study section.
2013 Reviewer NIH NIGMS Biomedical Technology Resource Centers P41 study section.
2013 - 2014 Special Issue Guest Editor for Sensors.
2011 - 2013 Reviewer SBIR/STTRBiological Chemistry and Biophysics NIH study section.
2008 Nanotechnology 50 award recipient for cell-free production of nanolipoprotein disc.
2005 Merck travel award, Radiation Oncology Gordon Conference.
2005 NIH scientific achievement award, Radiation Oncology Gordon Conference.
2004 - 2007 Lawrence Livermore National Laboratory Institutional Laboratory Directed Exploratory Research grant review committee.
2003 –2007 University of California's Industry-University Cooperative Research Program, Biotechnology Discovery grant review committee.
2001 - 2004 Protein Society Education Working Group.
2001 Research & Development 100 award recipient for “Gene Microdissection”.
1996 Stein Moore Graduate Student Award, Protein Society.
1995 - 1997 NIH, Molecular Biophysics Training Grant.
1994 Travel Award. Biology Department, Boston University.